Molecular Neuroendocrinology

Molecular Neuroendocrinology: From Genome to Physiology, provides researchers and students with a critical examination of the steps being taken to decipher genome complexity in the context of the expression, regulation and physiological functions of genes in neuroendocrine systems.

The 19 chapters are divided into four sectors: A) describes and explores the genome, its evolution, expression and the mechanisms that contribute to protein, and hence biological, diversity. B) discusses the mechanisms that enhance peptide and protein diversity beyond what is encoded in the genome through post-translational modification. C) considers the molecular tools that todays neuroendocrinologists can use to study the regulation and function of neuroendocrine genes within the context of the intact organism. D) presents a range of case studies that exemplify the state-of-the-art application of genomic technologies in physiological and behavioural experiments that seek to better understand complex biological processes.

 Written by a team of internationally renowned researchers
 Both print and enhanced e-book versions are available
 Illustrated in full colour throughout

This is the third volume in a new Series  Masterclass in Neuroendocrinology , a co- publication between Wiley and the INF (International Neuroendocrine Federation) that aims to illustrate highest standards and encourage the use of the latest technologies in basic and clinical research and hopes to provide inspiration for further exploration into the exciting field of neuroendocrinology.

Series Editors: John A. Russell, University of Edinburgh, UK and William E. Armstrong, The University of Tennessee, USA

Professor David Murphy, University of Bristol, UK
As part of The Molecular Neueroendocrinology Research Group, Professor Murphy uses gene discovery and transfer techniques to study the neuronal regulation of the cardiovascular system in health and disease.

Dr Harold Gainer, National Institute of Neurological Disorders and Stroke (NINDS), USA
Dr Gainer's research focuses on the mechanisms involved in the establishment and maintenance of specific peptidergic neuronal phenotypes in the central nervous system.

List of Contributors, vii

Series Preface, xi

About the Companion Website, xiii

Introduction 1
David Murphy and Harold Gainer

Part A Genome and Genome Expression

1 Evolutionary Aspects of Physiological Function and Molecular Diversity of the Oxytocin/Vasopressin Signaling System 5
Zita Liutkeviciute and Christian W. Gruber

2 The Neuroendocrine Genome: Neuropeptides and Related Signaling Peptides 25
J. Peter H. Burbach

3 Transcriptome Dynamics 57
David A. Carter, Steven L. Coon, Yoav Gothilf , Charles K. Hwang, Leming Shi, P. Michael Iuvone, Stephen Hartley, James C. Mullikin, Peter Munson, Cong Fu, Samuel J. Clokie, and David C. Klein

4 New Players in the Neuroendocrine System: A Journey Through the Noncoding RNA World 75
Yongping Wang, Edward A. Mead, Austin P. Thekkumthala, and Andrzej Z. Pietrzykowski

5 Transcription Factors Regulating Neuroendocrine Development, Function, and Oncogenesis 97
Judy M. Coulson and Matthew Concannon

6 Epigenetics 121
Chris Murgatroyd

Part B Proteins, Posttranslational Mechanisms, and Receptors

7 Proteome and Peptidome Dynamics 141
Lloyd D. Fricker

8 Neuropeptidomics: The Characterization of Neuropeptides and Hormones in the Nervous and Neuroendocrine Systems 155
Ning Yang, Samuel J. Irving, Elena V. Romanova, Jennifer W. Mitchell, Martha U. Gillette, and Jonathan V. Sweedler

9 Posttranslational Processing of Secretory Proteins 171
Nabil G. Seidah and Johann Guillemot

10 Neuropeptide Receptors 195
Stephen J. Lolait, James A. Roper, Georgina G.J. Hazell, Yunfei Li, Fiona J. Thomson, and AnneMarie OCarroll

Part C The Tool Kit

11 Germline Transgenesis 219
Jim Pickel

12 Somatic Transgenesis (Viral Vectors) 243
Valery Grinevich, H. Sophie KnoblochBollmann, Lena C. Roth, Ferdinand Althammer,

Andrii Domanskyi, Ilya A. Vinnikov, Marina Eliava, Megan Stanifer, and Steeve Boulant

13 Optogenetics Enables Selective Control of Cellular Electrical Activity 275
Ryuichi Nakajima, Sachiko Tsuda, Jinsook Kim, and George J. Augustine

14 NonMammalian Models for Neurohypophysial Peptides 301
Einav Wircer, Shifra BenDor, and Gil Levkowitz

Part D Case Studies Integration and Translation

15 Osmoregulation 331
David Murphy, Jose AntunesRodrigues, and Harold Gainer

16 Food Intake, Circuitry, and Energy Metabolism 355
Giles S.H. Yeo

17 Stress Adaptation and the HypothalamicPituitaryAdrenal Axis 375
Greti Aguilera

18 Neuroendocrine Control of Female Puberty: Genetic and Epigenetic Regulation 405
Alejandro Lomniczi and Sergio R. Ojeda

19 Oxytocin, Vasopressin, and Diversity in Social Behavior 423
Lanikea B. King and Larry J. Young

Glossary 443

Index 459